Dị ka njikọ nke amino acid na shuga, peptide sugar nwere ike kewaa ụzọ anọ: O glycosylation, C a N glycosylation, igirigi saccharification na GPI (glycophosphatidlyinositol).
1. N-glycosylation glycopeptides na-emi esịnede N-acetamide glucose na mbenata njedebe nke glycan yinye (Glc-Nac) jikọtara na N atom na otu amide nke n'akụkụ yinye nke ụfọdụ Asn na peptide yinye, na Asn. ike ijikọ agbụ glycan ga-adị na AsN-X-Ser / Thr (X! = P) n'ime motif guzobere site na residues.Sugar bụ N-acetylglucosamine.
N-glycosylation meziri glycopeptide nhazi
2. Ọdịdị nke O-glycosylation dị mfe karịa N-glycosylation.Glycopeptide a na-adịkarị mkpụmkpụ karịa glycan, mana nwere ụdị karịa N-glycosylation.Ser na Thr nwere ike ịbụ glycosylated n'ime yinye peptide.Na mgbakwunye, enwere glycopeptides ejiri tyrosine, hydroxyl, na hydroxyproline glycosylation chọọ mma.Ọnọdụ njikọ bụ hydroxyl oxygen atom nke dị n'akụkụ akụkụ nke ihe fọdụrụ.Ihe jikọrọ sugars bụ galactose ma ọ bụ N-acetylgalactosamine (Gal&GalNAc) ma ọ bụ glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Man/ManNAc), wdg.
O-glycosylation na-agbanwe nhazi ahụ
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
Otu N-acetylglucosamine (GlcNAc) glycosylation na-ejikọta proteins O-GlcNAc na hydroxyl oxygen atom nke serine ma ọ bụ threonine fọdụrụnụ nke protein.O-GlcNA glycosylation bụ ihe ịchọ mma O-GlcNAc monosaccharide na-enweghị ndọtị glycan;Dị ka peptide phosphorylation, O-GlcNAc glycosylation nke glycopeptides bụkwa usoro ịchọ mma protein siri ike.Ihe ịchọ mma O-GlcNAc na-adịghị mma nwere ike ibute ọrịa dị iche iche dịka ọrịa shuga, ọrịa obi, etuto ahụ, ọrịa Alzheimer na ndị ọzọ.
Isi ihe glycosylation nke glycopeptides
A na-ejikọta ihe ndị bụ isi nke polypeptide na agbụ shuga na agbụ protein site na njikọ covalent, na saịtị ndị na-ejikọta agbụ shuga ka a na-akpọ saịtị glycosylation.Ebe ọ bụ na ọ dịghị template iji soro biosynthesis nke glycopeptide sugar chains, a ga-ejikọta agbụ shuga dị iche iche n'otu ebe glycosylation, na-eduga na ihe a na-akpọ inhomogeneity microscopic.
Glycosylation nke glycopeptides
1. Mmetụta nke glycopeptide glycosylation na ọgwụgwọ-irụ ọrụ nke protein ọgwụgwọ.
N'ihe banyere protein ọgwụgwọ ọgwụgwọ, glycosylation na-emetụtakwa ọkara ndụ na ebumnuche nke ọgwụ protein na vivo.
2. Soluble glycopeptide glycosylation na protein
Nnyocha e mere egosiwo na agbụ shuga dị n'elu protein nwere ike imeziwanye ike nke protein
3. Glycopeptide glycosylation na protein immunogenicity
N'otu aka ahụ, agbụ shuga dị n'elu protein nwere ike ime ka ọ bụrụ azịza mgbochi ụfọdụ.N'aka nke ọzọ, agbụ shuga nwere ike kpuchie akụkụ ụfọdụ n'elu protein ma belata immunogenicity ya
4. Glycopeptide glycosylation nke na-eme ka nkwụsi ike protein dịkwuo elu
Glycosylation nwere ike ịbawanye nkwụsi ike nke protein na ọnọdụ denaturation dị iche iche (dịka denaturants, okpomọkụ, wdg) ma zere nchịkọta nke protein.N'otu oge ahụ, agbụ shuga dị n'elu ndị na-edozi ahụ nwekwara ike kpuchie ụfọdụ ihe mmebi proteolytic nke mkpụrụ ndụ protein, si otú ahụ na-abawanye nguzogide protein na proteinases.
5. Glycopeptide glycosylation nke na-emetụta ọrụ ndu nke ụmụ irighiri protein
Ịgbanwe glycosylation protein nwekwara ike ime ka ụmụ irighiri protein nwee ike imepụta ihe omume ọhụrụ
Oge nzipu: Ọgọst-03-2023