Integrin, ma ọ bụ integrin, bụ heterodimer transmembrane glycoprotein receptor nke na-emezi nrapanye sel anụmanụ na akara.Ọ mejupụtara yaα naβ subunits.Ọ na-etinye aka na njikarịcha nke omume cellular dị iche iche gụnyere mbugharị cell, ntinye sel, akara cell na intercellular, adhesion cell, na usoro angiogenesis.IntegrinαvβA na-enyocha 3 ugbu a karịa ebe niile.Ọdịdị nke integrinαvβ3 nwere njikọ chiri anya na mbugharị tumor, angiogenesis, mbufụt na osteoporosis.A na-egosipụta Integrin nke ukwuu na anụ ahụ tumor na akpụkpọ anụ endothelial nke neovascularization.Ọdịdị nke integrin nwere njikọ chiri anya na mbugharị tumor na angiogenesis.N'ime afọ ndị na-adịbeghị anya, ọmụmụ sayensị egosila na enwere 11 integrins nwere ike jikọta kpọmkwem na RGD peptide, nke bụ peptides na-emegide ndị na-anabata integrin.
A na-ekewa peptide RGD ka ọ bụrụ peptide RGD linear na RGD cyclic peptide.E jiri ya tụnyere peptide RGD linear, peptide cyclic RGD nwere ndakọrịta nnabata siri ike yana nkọwapụta nke nnabata.Ndị a bụ ụdị nkịtị na usoro njikọ nke RGD cyclic peptide.
Ụdị peptides cyclic RGD a na-ahụkarị:
1. peptides cyclic nwere usoro RGD mebere site na njikọ disulfide.
2. cyclic peptides nwere usoro RGD kpụrụ site na amide bond
Njikọ nke RGD cyclic peptide:
Ụdị ịba uru metụtara usoro njikọ peptide cyclic RGD n'ọhịa nke teknụzụ njikọ njikọ polypeptide siri ike.Usoro ọhụrụ bụ ịhọrọ resin 2-chloro-triphenylmethyl chloride dị ka onye na-ebu ihe achọrọ, buru ụzọ jikọọ ndị otu carboxyl nke mbụ n'akụkụ ya na otu nchebe pụrụ iche nke D aspartic acid amino acid, wee jikọọ peptide linear nke usoro peptide RGD na resin. , na amino acid ikpeazụ iji wepụ FMOC nchebe na-enweghị piperidine.Agbakwunyere ihe na-eme ka ọ bụrụ ihe a kapịrị ọnụ iji wepụ otu ihe nchebe carboxyl dị n'akụkụ nke mbụ D aspartic acid ozugbo site na resin, na-esote mgbakwunye piperidine iji wepụ otu FMOC na-echebe amino acid nke njedebe amino acid, na-esote mgbakwunye nke onye na-ejikọta ya. iji kpochapu ma kpochie otu carboxyl na amino group kpughere site na isi na njedebe nke peptide linear ozugbo site na resin n'ụdị amide bond iji mepụta peptide cyclic.N'ikpeazụ, a na-ebipụ peptide cyclic ozugbo site na resin na ngwọta ịkpụ.
Oge nzipu: Eprel-24-2023