Usoro ọhụrụ Thymus bụ nke 28 amino acid synthetic polypeptide regulator bụ ihe mejupụtara ya.Usoro Thymus bụ ụdị ọgwụ ọhụrụ uct, nke a na-ejikarị eme ihe na ndị ọrịa tumor, nwere ike ịkwalite mgbochi.
Usoro nke ime ihe
Amụbeghị nke ọma usoro thymalfasin na ọgwụgwọ ọrịa ịba ọcha n'anya B nke na-adịghị ala ala, mana nyocha dị iche iche egosila na thymalfasin nwere nzaghachi mgbochi dị ukwuu.A na-arụzu ya nke ọma site n'ịkwalite ọrụ cell T, ọ na-agakwa na steeti ndị gụnyere mmepụta cell T, nkewa na nhazigharị, na apoptosis.Site n'ịbawanye ọnụọgụ zuru oke nke lymphoid, egbu egbu anụ ahụ intrahepatic, CD3 na sel CD4, thymalfasin nwere ike ịrụ ọrụ immunomodulatory.Pleural fasin nwere ike ịkwalite maturation na nkewa nke sel DC, wee si otú a kwalite maturation na nkewa nke mkpụrụ ndụ T.Thymalfasin nwere ike ịgbalite mitogen ma ọ bụ antigen, mee ka mmepụta nke cytokines dị iche iche dị ka interferons mmadụ interleukin IL2 na IL3, na-eme ka nkwupụta nke interleukin-2 receptor dịkwuo elu, ma mee ka mkpụrụ ndụ CD4T dị iche na THLS, nke nwere mmetụta mgbochi nje.Site n'ịbawanye ọrụ nke mkpụrụ ndụ P, ọ nwere ike ịkwalite mmepụta nke lymphocytes na vivo, gbanwee nha nke P cell subsets na U cell iche, yana mmepụta nke ọgwụ nje, na-akpali mmepụta nke lymphokines ndị ọzọ antiviral na macrophages, kwalite ilo. na-arụ ọrụ, ma melite ọrụ nke usoro ahụ ji alụso ọrịa ọgụ na ndị ọrịa cancer.Ọ nwekwara ike wepụ nje bacteria, nje bacteria na nje n'ime ahụ.Akwụkwọ usoro Thymus maka usoro ụjọ ahụ na endothelium gbara ya gburugburu, bilie iji dozie ọrụ nke nnyefe hippocampal neuron synapti.Usoro Thymus ọhụrụ excitatory synaptik nnyefe na ụjọ usoro, nwere ike iji gbochie cancer ndị ọrịa kpatara ọrịa cancer dị ka chemotherapy na neurotoxicity.Pleuraxin nwere mmetụta nchịkwa mkpụrụ ndụ ihe nketa, nwere ike ime ka mkpụrụ ndụ ihe nketa kinase, mụbaa mkpụrụ ndụ ihe nketa cytokine, mkpụrụ ndụ ihe nketa na-ahụ maka nzaghachi mgbochi.Usoro nhazi nke thymalfasin na mkpụrụ ndụ ihe nketa IL na-egosi na thymalfasin na-akpali sel ndị na-eweta antigen."Ọzọkwa, mmetụta thymalfasin na uto cell nke na-alụso ọrịa ọgụ na-egosipụta na mmụba nke ihe na-eto eto cell na mkpụrụ ndụ ihe nketa hematopoietic."
Usoro ọhụrụ nke thymectomy
Usoro nke nkwadebe
1. Usoro mmịpụta biochemical na-enweta nkwadebe Thymosin (ngwakọta nke peptides) site na anụ ahụ anụmanụ, yabụ ọrụ ahụ dị nnọọ iche.N'ihi na ọ nwere protein anụmanụ, enwere ohere dị elu nke mgbaàmà nrịanrịa.Usoro thymus enwetara site na biochemically sitere na thymus embrayo mmadụ bụ nke ọhụrụ, na-enwe obere mkpụrụ mana ọ na-arụ ọrụ nke ọma.N'ịtụle akụrụngwa akụrụngwa nwere oke, ọnụ ahịa dị elu, mkpụrụ dị ala, yana usoro nnweta dị mgbagwoju anya., nnukwu nsogbu mmepụta ihe, atụmanya mmepe adịghị enwe nchekwube.
2. Ngwakọta kemịkalụ thymusfasin na-azụ ahịa bụ nke a na-emepụta nke ọma dị ka SPSS, nke a na-emepụta ma sachaa ya site na usoro kwesịrị ekwesị.Dabere na resin, a na-eji Fmoc mee ihe maka nhazi oge siri ike, na ụdị amino acid 28 ka emepụtara dịka thymalfasin si kwuo.N-terminal amino acid bụ acetic anhydride jiri acetylated, na TFA e ji mee ihe dị mkpa cracking reagent maka cracking iji nweta ike ike ngwaahịa.HPLC kewapụrụ ngwaahịa crude ahụ iji nweta ngwaahịa usoro thymus ọhụrụ, nke emeziri ka ọ dị ọcha iji nweta usoro akụrụngwa thymus ọhụrụ.
Enwere ụdị nje bacteria na-emepụta mkpụrụ ndụ atọ
1) Ọtụtụ mkpụrụ ndụ ihe nketa na-cloned n'ime onye ọbịa iji nweta lekwasịrị anya mkpụrụ ndụ ihe nketa, na n'ikpeazụ gosi n'ụdị nsonye ahu.A kọwapụtala na mkpụrụ ndụ ihe nketa nke thymethod bụ mkpụrụ ndụ ihe nketa multi-string sitere n'usoro nhụsianya na usoro PCR wee megharịa ya na Escherichia coli.(2) A na-ejikọta mkpụrụ ndụ ihe nketa protein fusion ahụ n'ime mgbasa ozi, ma gbanwee usoro mgbasa ozi ka ọ bụrụ onye ọbịa iji wuo nje bacteria na-emepụta mkpụrụ ndụ ihe nketa.Dịka ọmụmaatụ, dabere na mkpụrụ ndụ peptide mgbaàmà na plasmid, a na-ewu okwu plasmid, gbanwee ghọọ Pichia pastoris site na electroexcitation, mgbe ahụ, protein fusion sitere na methanol kpatara ya, mgbe ahụ, e ji usoro mgbaze enzyme na ụzọ ndị ọzọ rụọ ọrụ thymus ọhụrụ. .
Oge nzipu: Sep-08-2023