Ụzọ nke mmịpụta
N'afọ ndị 1950 na 1960, ọtụtụ mba dị n'ụwa, gụnyere China, na-ewepụtakarị peptides site na akụkụ anụmanụ.Dịka ọmụmaatụ, a na-akwado ogwu ogwu thymosin site n'igbu nwa ehi amụrụ ọhụrụ, wepụ thymus ya, wee jiri oscillating nkewa biotechnology kewapụ peptides na nwa ehi thymus.A na-eji thymosin a eme ihe n'ọtụtụ ebe iji mezie ma kwalite ọrụ mgbochi sel n'ime mmadụ.
A na-ekesa peptides eke bioactive n'ọtụtụ ebe.Enwere ọtụtụ peptides bioactive na anụmanụ, osisi na ihe ndị dị ndụ mmiri na okike, nke na-arụ ọrụ dị iche iche nke anụ ahụ ma na-ejigide ọrụ ndụ nkịtị.Ndị a peptides bioactive sitere n'okike gụnyere metabolites nke abụọ nke ihe dị ndụ dịka ọgwụ nje na homonụ, yana peptides bioactive dị na sistemu anụ ahụ dị iche iche.
Ka ọ dị ugbu a, ọtụtụ peptides bioactive kewapụrụ na mmadụ, anụmanụ, osisi, microbial na ihe ndị dị ndụ mmiri.Otú ọ dị, a na-ahụkarị peptides bioactive na obere ihe dị n'ime ihe ndị dị ndụ, na usoro dị ugbu a maka ikewapụ na sachapụ peptides bioactive site na ihe ndị sitere n'okike ezughị oke, yana ọnụ ahịa dị elu na obere bioactivity.
Ụzọ ndị a na-ejikarị eme ihe maka mmịpụta peptide na nkewa na-agụnye salting, ultrafiltration, gel filtration, isoelectric point precipitation, ion exchange chromatography, affinity chromatography, adsorption chromatography, gel electrophoresis, wdg. Isi mwepu ya bụ mgbagwoju anya nke ọrụ na ọnụ ahịa dị elu.
Usoro acid-base
A na-ejikarị acid na alkali hydrolysis eme ihe na ụlọ ọrụ nnwale, mana a naghị ejikarị eme ihe na mmepụta ihe.Na usoro nke alkaline hydrolysis nke protein, ọtụtụ amino asịd dị ka serine na threonine na-ebibi, racemization emee, na ọnụ ọgụgụ buru ibu nke nri na-efunahụ.Ya mere, a na-ejikarị usoro a eme ihe na mmepụta.Acid hydrolysis nke protein anaghị eme ka agbụrụ nke amino asịd, hydrolysis bụ ngwa ngwa na mmeghachi omume zuru ezu.Agbanyeghị, ọghọm ya bụ teknụzụ dị mgbagwoju anya, njikwa siri ike na mmetọ gburugburu ebe obibi siri ike.Nkesa arọ molekụla nke peptides bụ ihe na-ezighi ezi na enweghị ntụkwasị obi, yana ọrụ physiological ha siri ike ịchọpụta.
Enzymatic hydrolysis
A na-ahụ ọtụtụ peptides bioactive na ogologo agbụ nke protein na steeti anaghị arụ ọrụ.Mgbe otu protease mere hydrolyzed, a na-ahapụ peptide ha na-arụ ọrụ na usoro amino nke protein.Mwepụta Enzymatic nke peptides bioactive sitere na anụmanụ, osisi na ihe ndị dị n'ime mmiri abụrụla ihe nyocha lekwasịrị anya n'ime iri afọ ndị na-adịbeghị anya.
Enzymatic hydrolysis nke peptides bioactive bụ nhọrọ nke protease kwesịrị ekwesị, na-eji protein dị ka ihe na-edozi ahụ na protein hydrolyzing iji nweta ọnụ ọgụgụ buru ibu nke peptides bioactive nwere ọrụ dị iche iche physiological.N'ime usoro mmepụta, okpomọkụ, uru PH, ntinye enzyme, ntinye ntinye na ihe ndị ọzọ nwere njikọ chiri anya na mmetụta enzymatic hydrolysis nke obere peptides, na isi ihe bụ nhọrọ nke enzyme.N'ihi enzymes dị iche iche a na-eji maka enzymatic hydrolysis, nhọrọ na nhazi nke enzymes, na protein protein dị iche iche, peptides na-esi na ya pụta dịgasị iche iche na oke, nkesa molecular arọ, na amino acid mejupụtara.Mmadụ na-ahọrọkarị proteases anụmanụ, dị ka pepsin na trypsin, na proteases osisi, dị ka bromelain na papain.Site na mmepe nke sayensị na teknụzụ yana ihe ọhụrụ na-aga n'ihu nke teknụzụ enzyme dị ndụ, a ga-achọpụtakwu enzymes ma jiri ya mee ihe.A na-eji enzyme hydrolysis eme ihe n'ọtụtụ ebe na nkwadebe nke peptides bioactive n'ihi nkà na ụzụ tozuru okè na ntinye ego dị ala.
Oge nzipu: Mee-30-2023