Kedu ọrụ nke phosphorylation na peptides?

Phosphorylation na-emetụta akụkụ niile nke ndụ cell, na protein kinase na-emetụta akụkụ niile nke ọrụ nkwurịta okwu intracellular site n'ịhazi ụzọ mgbaàmà na usoro cellular.Otú ọ dị, aberrant phosphorylation bụkwa ihe kpatara ọtụtụ ọrịa;karịsịa, protein kinases na phosphatases mutated nwere ike ịkpata ọtụtụ ọrịa, na ọtụtụ nsị eke na pathogens nwekwara mmetụta site n'ịgbanwe ọnọdụ phosphorylation nke protein intracellular.

Phosphorylation nke serine (Ser), threonine (Thr), na tyrosine (Tyr) bụ usoro mgbanwe protein nwere ike ịgbanwe.Ha na-etinye aka na nhazi nke ọtụtụ ọrụ cellular, dị ka akara ngosi nnabata, njikọ protein na nkewa, ịgbalite ma ọ bụ igbochi ọrụ protein, na ọbụna ndụ ndụ.A na-ebo phosphates ebubo na-adịghị mma (ụgwọ abụọ na-adịghị mma maka otu phosphate).Ya mere, mgbakwunye ha na-agbanwe ihe onwunwe nke protein, nke na-abụkarị mgbanwe mgbanwe, na-eduga n'ịgbanwe nhazi nke protein.Mgbe ewepụrụ otu phosphate, ngbanwe nke protein ahụ ga-alaghachi na ọnọdụ mbụ ya.Ọ bụrụ na protein abụọ ahụ na-arụkọ ọrụ ọnụ na-egosipụta ọrụ dị iche iche, phosphorylation nwere ike ịrụ ọrụ dị ka mgbanwe molekụla maka protein iji chịkwaa ọrụ ya.

Ọtụtụ homonụ na-achịkwa ọrụ nke enzymes kpọmkwem site n'ịbawanye ọnọdụ phosphorylation nke serine (Ser) ma ọ bụ threonine (Thr) residues, na tyrosine (Tyr) phosphorylation nwere ike ịkpalite site na ihe uto (dị ka insulin).Enwere ike iwepụ otu phosphate nke amino acid ndị a ngwa ngwa.Ya mere, Ser, Thr, na Tyr na-arụ ọrụ dị ka mgbanwe molekụla na nhazi nke ihe omume cellular dị ka mmụba tumor.

Ndị peptides sịntetị na-arụ ọrụ bara uru nke ukwuu n'ịmụ ihe gbasara protein kinase substrates na mmekọrịta.Otú ọ dị, e nwere ụfọdụ ihe na-egbochi ma ọ bụ na-amachi mgbanwe nke phosphopeptide synthesis technology, dị ka enweghị ike nweta zuru automation nke siri ike-phase njikọ na enweghị adaba njikọ na ọkọlọtọ nyocha nyiwe.

Ngwakọta peptide nke dabeere na ikpo okwu na nkà na ụzụ mgbanwe phosphorylation na-emeri njedebe ndị a ma na-emeziwanye arụmọrụ na scalability, na ikpo okwu dabara nke ọma maka ọmụmụ nke protein kinase substrates, antigens, molecules molecules, and inhibitors.