Methylation-modified peptides, nke a makwaara dị ka peptides methylation, bụ ihe ịchọ mma post-translational (PTMs) ma na-arụ ọrụ nchịkwa dị mkpa n'ihe fọrọ nke nta ka ọ bụrụ ọrụ niile nke ndụ na sel.Methyltransferase na-eme ka ndị na-edozi ahụ nwee ike ibufe otu hydroxyl gaa na amino acid dị iche iche maka njikọta covalent.Methylation bụ usoro mgbanwe mgbanwe site na demethylases.
Nnyocha egosiwo na amino acid methylated/demethylated na-abụkarị lysine (Lys) na arginine (Arg).Nnyocha achọpụtala na histone lysine methylation nwere ọrụ dị iche iche dị ndụ dị ka nhazi cell cell na nkewa, X chromosome inactivation, ụkpụrụ ntụgharị na mmeghachi omume DNA.", na-emetụtakarị condensation chromatin ma na-egbochi okwu mkpụrụ ndụ ihe nketa."Histone arginine methylation na-arụ ọrụ dị mkpa na nhazi nke mkpụrụ ndụ ihe nketa ma nwee ike imetụta usoro ọmụmụ ihe dị iche iche na sel, gụnyere nrụzi DNA, ntụgharị mgbaàmà, mmepe cell, na carcinogenesis.Ya mere, Guopeptide Biology ewepụtala teknụzụ nke methyl decorative peptides, nke ndị ọkà mmụta sayensị gbanwere mgbe nsụgharị protein (PTMS) gasịrị iji nyere aka na nyocha.
Mgbanwe Methylation (Me1, Me2, Me3)
Fmoc-Lys dị elu (M, Boc) -OH, Fmoc-Lys (Me2) -OH, Fmoc-Lys (Me3) -OH.HCL, Fmoc-Arg (Me, Pbf) -OH, Fmoc-Arg (M) 2-OH.HCl (asymmetrical), F na-eji moc-Arg (me) 2-OH.HCl (symmetrical) na akụrụngwa ndị ọzọ sitere na FMOC siri ike usoro nhazi usoro iji nweta Lys na Arg methylated peptides, na ngwaahịa ndị ahụ. bụ HPLC sachara.A na-enye spectra mass dị mkpa, HPLC chromatogram na COA maka ngwaahịa emechara.
Oge nzipu: Nov-24-2023